Non-Bias-Limited Tracking of Spherical Particles, Enabling Nanometer Resolution at Low Magnification
نویسندگان
چکیده
منابع مشابه
Non-bias-limited tracking of spherical particles, enabling nanometer resolution at low magnification.
We present a three-dimensional tracking routine for nondiffraction-limited particles, which significantly reduces pixel bias. Our technique allows for increased resolution compared to that of previous methods, especially at low magnification or at high signal/noise ratio. This enables tracking with nanometer accuracy in a wide field of view and tracking of many particles. To reduce bias induced...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2012.03.073